Improved Method of Enzyme Digestion for Root Tip Cytology

Enzyme Cytology

For many years, cytologists have trudged down pathways into the fertile valley of the cell organelles. Nowadays, investigators, mainly biochemists, traverse a superhighway into the same valley and, while there is a confluence of the pathways and the superhighway, much still depends on the position of the observer and his lingo. Although it is positively ecumenical to find a morphologist referri...

Separation of lentil root tip chromosomes by colchicine

A Recyclable Poly(ionic liquid)s Enzyme Reactor for Highly Efficient Protein Digestion

One of the most significant tasks for proteomic research and industrial applications, is the preparation of recyclable enzyme reactor. Herein, a novel recyclable enzyme reactor has been developed based on monodispersed spherical poly(quaternary ammonium ionic liquid)s particles immobilized trypsin. A new quaternary ammonium ionic liquids functional monomer was first synthesized. The ionic l...

An improved HPLC method for determination of colocynthin in colocynth

Background and objectives: Colocynthin is the major active secondary metabolite of colocynth, Citrullus colocynthis (L.) Schrad, which has been used in traditional and ethno medicine of many countries.  It could be considered as an active marker for quality control of colocynth and its herbal products. Analysis and standardization of colocynth and its herbal preparations are a critical...

Validation of eco-enzyme for improved water quality effect during large public gathering at river bank

 In contrast to existing trends and expected deteriorating water quality in the river Yamuna in Delhi which carries potential cultural economical and health importance, March 2016 water data showing improvement despite large public gathering at river bank during Cultural Festival. The reasons and factors were investigated. The paper presents the test reports of eco- enzyme which was found to be...